Nine compounds bearing pyridinyl (or piperidinyl, benzimidazolyl, benzotriazolyl) groups\nbound to an azelayl moiety through an amide bond were synthesized. The structural analogy with\nsome histone deacetylase inhibitors inspired their syntheses, seeking new selective histone deacetylase\ninhibitors (HDACi). The azelayl moiety recalls part of 9-hydroxystearic acid, a cellular lipid showing\nantiproliferative activity toward cancer cells with HDAC as a molecular target. Azelayl derivatives\nbound to a benzothiazolyl moiety further proved to be active as HDACi. The novel compounds\nwere tested on a panel of both normal and tumor cell lines. Non-specific induction of cytotoxicity\nwas observed in the normal cell line, while three of them induced a biological effect only on the\nosteosarcoma (U2OS) cell line. One of them induced a change in nuclear shape and size. Cell-cycle\nalterations are associated with post-transcriptional modification of both H2/H3 and H4 histones.\nIn line with recent studies, revealing unexpected HDAC7 function in osteoclasts, molecular docking\nstudies on the active molecules predicted their proneness to interact with HDAC7. By reducing side\neffects associated with the action of the first-generation inhibitors, the herein reported compounds,\nthus, sound promising as selective HDACi
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